Volume 2, Issue 6, November 2016, Page: 101-106
Translocator Protein 18 kDa Involved in the Cognitive Impairment Induced by Isoflurane Inhalation Anesthesia
Rui Zhang, Department of Anesthesiology, Weifang Medical University, Weifang, China
Shanshan Zou, Department of Anesthesiology, Weifang Medical University, Weifang, China
Lingzhong Meng, Department of Anesthesiology, Yale University Medical School, USA
Ming Ding, Department of Anesthesiology, No.89 Hospital of People’s Liberation Army, Weifang, China
Huirong Han, Department of Anesthesiology, Weifang Medical University, Weifang, China
Received: Aug. 23, 2016;       Accepted: Sep. 6, 2016;       Published: Oct. 14, 2016
DOI: 10.11648/j.ijcems.20160206.11      View  3521      Downloads  94
Background. Translocator protein 18 kDa (TSPO) plays a key role both in microglial activation and neuroinflammation. Postoperative cognitive decline (POCD), a notable hazard to both patients and society, maybe contribute to deficiently controlled neuroinflammatory processes initiated by anesthesia and surgery. So far it is unclear that whether TSPO is involved in the pathogenesis of POCD or not. Materials and Methods. Twelve adult rats and twelve aged rats were divided into control and isoflurane groups respectively. POCD was induced by a 4-hour exposure of 2% isoflurane. The memory retention capability was assessed by the Morris water maze trial, the mRNA and the protein expression of both TSPO and Iba1 were assessed by real-time quantitative PCR and Western Blot analysis separately. Results. Compared to the control group, the latency time to find platform was longer in the groups exposed to isoflurane (p<0.05); the mRNA and the protein expression of both TSPO and Iba1 were correspondingly upregulated (p<0.05); especially that the severity of cognitive decline and the degree of TSPO and Iba1 over-expression were significantly different between the adult and aged rats (p<0.05); The twice times across the platform showed no significant difference among all the groups. Conclusions. Our study for the first time showed that TSPO may be involved in the pathogenesis of the cognitive decline induced by isoflurane anesthesia. Its role for being a biomarker and an interventional target of POCD deserves future investigation.
Postoperative Cognitive Decline (POCD), Translocator Protein 18 kDa (TSPO), Neuroinflammation
To cite this article
Rui Zhang, Shanshan Zou, Lingzhong Meng, Ming Ding, Huirong Han, Translocator Protein 18 kDa Involved in the Cognitive Impairment Induced by Isoflurane Inhalation Anesthesia, International Journal of Clinical and Experimental Medical Sciences. Vol. 2, No. 6, 2016, pp. 101-106. doi: 10.11648/j.ijcems.20160206.11
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