Volume 4, Issue 2, March 2018, Page: 21-25
Effect of Aging on the Progression of Articular Cartilage Damage in a Rat Osteoarthritis Model
Masanori Watanabe, Department of Physical Therapy, Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan
Satoshi Kojima, Course of Rehabilitation, Graduate School of Rehabilitation, Kinjo University, Hakusan, Japan
Keiji Asada, Department of Physiotherapy, Faculty of Health Science, Suzuka University of Medical Science, Suzuka, Japan
Itaru Hibino, Department of Physical Therapy, Faculty of Rehabilitation Science, Nagoya Gakuin University, Seto, Japan
Masahiro Hoso, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan
Received: Mar. 28, 2018;       Accepted: Apr. 17, 2018;       Published: May 10, 2018
DOI: 10.11648/j.ijcems.20180402.11      View  1308      Downloads  74
This study aimed to investigate and clarify the effect of aging on the progression of cartilage damage using a rat osteoarthritis model. In total, 12 rats, including three rats per age group, were assigned to the experimental group, and two rats (one 3 and 18 months old each) were assigned to the control group. In the experimental group, joint instability was induced using the destabilization of the medial meniscus model. After completion of the 4-week experimental period, the bilateral knee joints were harvested and the histological changes in the articular structures of the joints were observed using a light microscope. Medial dislocation of the medial meniscus, low staining of the cartilaginous substrate on the tibial side localized around the medial meniscus tip, and irregularities and fibrillation of the superficial layer of the cartilage on the tibial side were observed in all experimental age groups. No significant age-related differences were observed in the degree of observed findings and the score of cartilage degeneration. The results indicate that aging does not affect the early stages of progression of cartilage damage.
Osteoarthritis, Aging, Histopathology
To cite this article
Masanori Watanabe, Satoshi Kojima, Keiji Asada, Itaru Hibino, Masahiro Hoso, Effect of Aging on the Progression of Articular Cartilage Damage in a Rat Osteoarthritis Model, International Journal of Clinical and Experimental Medical Sciences. Vol. 4, No. 2, 2018, pp. 21-25. doi: 10.11648/j.ijcems.20180402.11
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Woolf AD, Pfleger B. Burden of major musculoskeletal conditions. Bull World Health Organ. 2003; 81:646–56.
WHO. Chronic Diseases and Health Promotion. http://www.who.int/chp/topics/rheumatic/en/(Accessed Jan. 11, 2018)
Yoshimura N: Epidemiology of osteoarthritis in Japan; the ROAD study. Clin Calcium. 2011; 21:821-825 (in Japanese)
Ministry of Health, Labour and Welfare. Comprehensive Survey of Living Conditions. http://www.mhlw.go.jp/toukei/saikin/hw/k-tyosa/k-tyosa16/index.html (Accessed Jan. 11, 2018)
Badlani JT, Borrero C, Golla S, Harner CD, Irrgang JJ, et al.: The effects of meniscus injury on the development of knee osteoarthritis: data from the osteoarthritis initiative. Am J Sports Med. 2013; 41: 1238-1244.
Englund M, Guermazi A, Roemer FW, et al.: Meniscal tear in knees without surgery and the development of radiographic osteoarthritis among middle-aged and elderly persons: The Multicenter Osteoarthritis Study. Arthritis Rheum. 2009; 60: 831-839.
Rahmati M, Nalesso G, Mobasheri A, et al.: Aging and osteoarthritis: Central role of the extracellular matrix. Ageing Res Rev. 2017; 40: 20-30.
Loeser RF: Age-related changes in the musculoskeletal system and the development of osteoarthritis. Clin Geriatr Med. 2010; 26: 371-386.
Shane Anderson A, Loeser RF: Why is osteoarthritis an age-related disease? Best Pract Res Clin Rheumatol. 2010; 24: 15-26.
Lampropoulou-Adamidou K, Lelovas P, Karadimas EV, et al.: Useful animal models for the research of osteoarthritis. Eur J Orthop Surg Traumatol. 2014; 24: 263-271.
Kamekura S, Hoshi K, Shimoaka T, Chung U, et al.: Osteoarthritis development in novel experimental mouse models induced by knee joint instability. Osteoarthritis Cartilage. 2005; 13: 632-641.
Galsson SS, Blanchet TJ, Morris EA: The surgical destabilization of the model meniscus (DMM) model of osteoarthritis in the 129/SvEv mouse. Osteoarthritis Cartilage. 2007; 15: 1061-1069.
Glasson SS, Askew R, Sheppard B, et al.: Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature. 2005; 434: 644-648.
Watanabe M, Kojima S, Asada K, et al.: Comparison the rat models of osteoarthritis for examining the effect of the physical therapy. Journal of Nagoya Gakuin University, Medical, Health, and Sports Sciences (in Japanese) (in press).
Gerwin N, Bendele AM, Glasson S, et al.: The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat. Osteoarthritis Cartilage. 2010; 18: S24-34.
Ferrándiz ML, Terencio MC, Ruhí R, et al.: Influence of age on osteoarthritis progression after anterior cruciate ligament transection in rats. Exp Gerontol. 2014; 55: 44-48.
Moriyama H, Kanemura N, Brouns I, et al.: Effects of aging and exercise training on the histological and mechanical properties of articular structures in knee joints of male rat. Biogerontology. 2012; 13: 369-381.
Kuramoto K, Shumiya S, Inoue T I, et al.: Pathological findings of the rats naturally died during aged animal rearing. Biomedical Gerontology. 1986; 10: 71-72 (in Japanese).
Andreollo NA, Santos EF, Araújo MR, et al.: Rat's age versus human's age: what is the relationship? Arq Bras Cir Dig. 2012; 25: 49-51.
Sengupta P.: The Laboratory Rat: Relating Its Age With Human's. Int J Prev Med. 2013; 4: 624-630.
Barrientos RM, Watkins LR, Rudy JW, et al.: Characterization of the sickness response in young and aging rats following E. coli infection. Brain Behav Immun. 2009; 23: 450-454.
Boguszewski P, Zagrodzka J. Emotional changes related to age in rats--a behavioral analysis. Behav Brain Res. 2002; 133: 323-332.
Browse journals by subject